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Institutt for medisinsk biologi

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Artikler, rapporter og annet (medisinsk biologi) [84]
Mastergradsoppgaver i biologi (Helsefak) [20]
Mastergradsoppgaver i medisinske laboratoriefag [0]
Mastergradsoppgaver i molekylær bioteknologi [5]

Recent additions

Sex hormones and gene expression signatures in peripheral blood from postmenopausal women - the NOWAC postgenome study

Waaseth, Marit; Olsen, Karina Standahl; Rylander, Charlotta; Lund, Eiliv; Dumeaux, Vanessa (Journal article; Tidsskriftartikkel; Peer reviewed, 2011)

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URI: http://hdl.handle.net/10037/3815

File(s) in this item: 1

article.pdf (532.8Kb)

Effects of exogenous hydrogen sulfide administration on cardiac function and reactive oxygen species production : a study in hearts from normal rats and rats with heart hypertrophy or ischemia

Paunas, Teodora Ioana (Master thesis; Mastergradsoppgave, Sep-2011)

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Abstract:

Coronary heart disease is the leading cause of death worldwide. Infarct size can be limited by interventions used after the ischemic event like the use of thrombolytic therapy or primary percutaneous coronary intervention. Paradoxically, however, the return of blood flow can also result in additional cardiac damage and complications, referred to as reperfusion injury. It has been shown that reperfusion injuries can be decreased by postconditioning- rapid intermittent interruptions of blood flow in the early phase of reperfusion, or post-treatment using various drug therapies which applied during reperfusion can reduce infarct size. H2S, a gas that is synthesized in mammalian tissue, has been reported to be cardioprotective during ischemia-reperfusion injury. The means by which H2S is cardioprotective during I/R are believed to be: the opening of the sarcolemmal KATP channel, the generation of antiapoptotic effects inside the cells as well as a direct antioxidant effect. Low levels of reactive oxygen species (ROS) are constantly produce within cells and play important roles in cell signaling, cellular homeostasis, differentiation and apoptosis. However an excessive increase in the level of ROS can be harmful and has been proposed to play crucial roles or contribute in the development of various diseases. The aim of our study was to investigate the effects of H2S in an acute ischemia-reperfusion model and to determine whether exogenous administration of H2S in both healthy rats and rats exposed to experimental models of cardiac disease influenced the production of ROS. In order to do this we established a method trough which we were able to measure the presence of ROS in heart tissue samples harvested from normal rats and rats with heart hypertrophy and ischemic heart disease.

URI:

http://hdl.handle.net/10037/3726

File(s) in this item: 1

thesis.pdf (1.535Mb)

In HPA 1a-immunized women the decrease in anti-HPA 1a antibody level during pregnancy is not associated with anti-idiotypic antibodies

Kjeldsen-Kragh, J; Killie, Mette Kjær; Kim, Michael; Husebekk, Anne; Freedman, J; Semple, John W (Journal article; Tidsskriftartikkel, 2009)

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URI: http://hdl.handle.net/10037/3644

File(s) in this item: 1

article.pdf (638.9Kb)

Biosynthesis of Promatrix Metalloproteinase-9/Chondroitin Sulphate Proteoglycan Heteromer Involves a Rottlerin-Sensitive Pathway

Malla, Nabin; Berg, Eli; Moens, Ugo; Uhlin-Hansen, Lars; Winberg, Jan-Olof (Journal article; Tidsskriftartikkel; Peer reviewed, 2011)

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Abstract:

Background: Previously we have shown that a fraction of the matrix metalloproteinase-9 (MMP-9) synthesized by the macrophage cell line THP-1 was bound to a chondroitin sulphate proteoglycan (CSPG) core protein as a reduction sensitive heteromer. Several biochemical properties of the enzyme were changed when it was bound to the CSPG.
Methodology/Principal Findings: By use of affinity chromatography, zymography, and radioactive labelling, various macrophage stimulators were tested for their effect on the synthesis of the proMMP-9/CSPG heteromer and its components by THP-1 cells. Of the stimulators, only PMA largely increased the biosynthesis of the heteromer. As PMA is an activator of PKC, we determined which PKC isoenzymes were expressed by performing RT-PCR and Western Blotting. Subsequently specific inhibitors were used to investigate their involvement in the biosynthesis of the heteromer. Of the inhibitors, only Rottlerin repressed the biosynthesis of proMMP-9/CSPG and its two components. Much lower concentrations of Rottlerin were needed to reduce the amount of CSPG than what was needed to repress the synthesis of the heteromer and MMP-9. Furthermore, Rottlerin caused a minor reduction in the activation of the PKC isoenzymes d, e, h and u (PKD3) in both control and PMA exposed cells.
Conclusions/Significance: The biosynthesis of the proMMP-9/CSPG heteromer and proMMP-9 in THP-1 cells involves a Rottlerin-sensitive pathway that is different from the Rottlerin sensitive pathway involved in the CSPG biosynthesis. MMP-9 and CSPGs are known to be involved in various physiological and pathological processes. Formation of complexes may influence both the specificity and localization of the enzyme. Therefore, knowledge about biosynthetic pathways and factors involved in the formation of the MMP-9/CSPG heteromer may contribute to insight in the heteromers biological function as well as pointing to future targets for therapeutic agents.

URI:

http://hdl.handle.net/10037/3638

File(s) in this item: 1

article.pdf (2.009Mb)

Study of interaction between BK virus large T-antigen and agnoprotein

Adou, Koman Mireille Sophie Chinan (Master thesis; Mastergradsoppgave, Aug-2011)

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Abstract:

Human polyomavirus BK (BKV) is a non enveloped virus with a double-stranded, circular DNA genome. BKV infects >70% of the human population world-wide. Infection occurs predominantly during childhood and the virus remains in a latent state throughout life in the immune competent individuals. In the context of immunosuppression, however, reactivation occurs and can lead to renal stenosis and interstitial nephritis in kidney transplant patients, and hemorrhagic cystitis in bone narrow transplant patients. Moreover, BKV has been associated with several human cancers, but its causal role remains disputed. One of BKV’s protein known as agnoprotein may play a role in these pathogenic processes. To develop antiviral therapy it is required to elucidate the exact biological function of this protein. One way to examine the function of agnoprotein is by identifying possible cellular interaction partners. Another way is to understand agnoprotein’s role in the viral life cycle. Thereto, we examined the interaction of agnoprotein with another viral protein, large T-antigen (LT-ag) and the functional implication of this interaction. First, we investigated the effect of agnoprotein on the transcriptional activity of LT-ag on the BKV early promoter by transient transfection studies in HEK293. Our results revealed that LT-ag affects BKV early promoter in a concentration-dependent manner with low concentrations of LT-ag inhibiting, while high concentrations stimulated BKV early promoter activity. Co-expression of agnoprotein repressed LT-ag-induced activation of the BKV early promoter, suggesting that agnoprotein may exert a negative regulatory effect on transactivation by LT-ag. To test whether agnoprotein mediates its effect through direct interaction with LT-ag, we studied a possible association between these proteins. GST pulldown, co-immunoprecipitation (in vivo and in vitro), and mammalian two hybrid studies confirmed an interaction between LT-ag and agnoprotein.

URI:

http://hdl.handle.net/10037/3568

File(s) in this item: 1

thesis.pdf (2.113Mb)

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Institutt for medisinsk biologi

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