English
norskBlar i forfatter "Dalmo, Roy Ambli"
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Review on Immersion Vaccines for Fish: An Update 2019
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-11-29)Immersion vaccines are used for a variety of aquacultured fish to protect against infectious diseases caused by bacteria and viruses. During immersion vaccination the antigens are taken up by the skin, gills or gut and processed by the immune system, where the resulting response may lead to protection. The lack of classical secondary responses following repeated immersion vaccination may partly be ... -
Scavenger endothelial cells of fish, a review
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-05-17)The definition of scavenger endothelial cells (SEC) is exclusively based on functional and structural characteristics. The following characteristics are common hallmarks for the vertebrate SEC: (a) All vertebrates examined are furnished with a population of special SEC that plays a role in the catabolism of physiologic and non-physiologic soluble waste macromolecules. (b) From the ligands that are ... -
Strategies and hurdles using DNA vaccines to fish
(Journal article; Tidsskriftartikkel; Peer reviewed, 2014) -
Studies on the antibody response and side effects after intramuscular and intraperitoneal injection of Atlantic lumpfish (Cyclopterus lumpus L.) with different oil-based vaccines
(Journal article; Tidsskriftartikkel; Peer reviewed, 2017-05-26)Atlantic lumpfish (<i>Cyclopterus lumpus</i> L.) is used as a biological delousing agent for sea lice (<i>Lepeophtheirus salmonis</i> K.) infestations in Norwegian aquaculture. Here, we present a study on the antibody response and vaccine side effects after intramuscular and intraperitoneal injection of lumpfish with two vaccines. Both vaccines contained bacterial antigens from atypical <i>Aeromonas ... -
Studies on the effects of LPS, ß‐glucan and metabolic inhibitors on the respiratory burst and gene expression in Atlantic salmon macrophages
(Journal article; Tidsskriftartikkel; Peer reviewed, 2018-03-30)Reactive oxygen species (ROS) production in macrophage‐like cells is induced as an antimicrobial defence against invading pathogens. In this study, we have explored how different stimuli and metabolic inhibitors affect the level of respiratory burst in Atlantic salmon (<i>Salmo salar</i> L.) head kidney macrophage‐like cells. Cells stimulated in vitro by bacterial lipopolysaccharide (LPS) and ß‐glucan ... -
T-box transcription factor eomesodermin/Tbr2 in Atlantic cod (Gadus morhua L.): Molecular characterization, promoter structure and function analysis
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-07-11)Eomesodermin (Eomes) is a member of T-box transcription factor family and plays an important role in the regulation of a wide variety of developmental processes and immune response in animals. Here we report cloning and characterization of the full-length cDNA of Atlantic cod Eomes (GmEomes), which possesses a TBOX_3 domain similar to its counterpart in mammals. The regulated expression was observed ... -
Th17 master transcription factors RORα and RORγ regulate the expression of IL-17C, IL-17D and IL-17F in Cynoglossus semilaevis
(Journal article; Tidsskriftartikkel; Peer reviewed, 2015-11-10)The RAR-related orphan receptors (RORs) are members of the nuclear receptor family of intracellular transcription factors. In this study, we examined the regulatory properties of RORα (CsRORα) and RORγ (CsRORγ) in tongue sole (Cynoglossus semilaevis). CsRORα and CsRORγ expression was detected in major lymphoid organs and altered to significant extents after bacterial and viral infection. CsRORα ... -
Trained Innate Immunity of Fish Is a Viable Approach in Larval Aquaculture
(Journal article; Tidsskriftartikkel; Peer reviewed, 2019-01-25)The general understanding has been that only adaptive immunity is capable of immunological memory, but this concept has been challenged in recent years by studies showing that innate immune systems can mount resistance to reinfection—as the innate immune system can adapt its function following an insult. Innate immune training offers an attractive approach in intensive fish larval rearing, especially ... -
Transcription factor T-bet in Atlantic salmon: characterization and gene expression in mucosal tissues during Aeromonas salmonicida infection
(Journal article; Tidsskriftartikkel; Peer reviewed, 2015-07-06)The T-box transcription factor T-bet is expressed in a number of hematopoietic cell types in mammals and plays an essential role in the lineage determination of Th1 T-helper cells and is considered as an essential feature for both innate and adaptive immune responses in higher vertebrates. In the present study, we have identified and characterized the full-length Atlantic salmon T-bet cDNA (3502 ... -
Transgene and immune gene expression following intramuscular injection of Atlantic salmon (Salmo salar L.) with DNA-releasing PLGA nano- and microparticles
(Journal article; Tidsskriftartikkel; Peer reviewed, 2013)The use of poly-(D,L-lactic-co-glycolic) acid (PLGA) particles as carriers for DNA delivery has received considerable attention in mammalian studies. DNA vaccination of fish has been shown to elicit durable transgene expression, but no reports exist on intramuscular administration of PLGA-encapsulated plasmid DNA (pDNA). We injected Atlantic salmon (Salmo salar L.) intramuscularly with a plasmid ... -
Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)
(Journal article; Tidsskriftartikkel; Peer reviewed, 2021-04-12)Quantifying small, rapidly evolving forces generated by cells is a major challenge for the understanding of biomechanics and mechanobiology in health and disease. Traction force microscopy remains one of the most broadly applied force probing technologies but typically restricts itself to slow events over seconds and micron-scale displacements. Here, we improve >2-fold spatially and >10-fold temporally ... -
Vaccination of Atlantic lumpfish (Cyclopterus lumpus L.) at a low temperature leads to a low antibody response against Aeromonas salmonicida
(Journal article; Tidsskriftartikkel; Peer reviewed, 2017-12-11)We present a study on the effect of water temperature on immunization of Atlantic lumpfish. In total, 360 fish were vaccinated with either 50 μl of an oil‐based injection vaccine (VAX), with Aeromonas salmonicida and Vibrio salmonicida antigens, or PBS. Fish were vaccinated at three different water temperatures, 5°C, 10°C and 15°C, and sorted into six groups (N = 60). Lumpfish were weighed every 3 ... -
Vaccine Adjuvants Induce Formation of Intraperitoneal Extracellular Traps in Flounder (Paralichthys olivaceus)
(Journal article; Tidsskriftartikkel; Peer reviewed, 2022-03-30)Adjuvants are used to increase the strength, quality, and duration of the immune response of vaccines. Neutrophils are the first immune cells that arrive at the injection site and can release DNA fibers together with granular proteins, so-called neutrophil extracellular traps (NETs), to entrap microbes in a sticky matrix of extracellular chromatin and microbicidal agents. Similar extracellular ... -
Vaccines and Immunostimulants for finfish
(Journal article; Tidsskriftartikkel; Peer reviewed, 2020-09-29)Following a successful renal transplantation circulating markers of inflammation may remain elevated, and systemic inflammation is associated with worse clinical outcome in renal transplant recipients (RTRs). Vitamin D-receptor (VDR) activation is postulated to modulate inflammation and endothelial function. We aimed to explore if a synthetic vitamin D, paricalcitol, could influence systemic ...
